0.25ml 0.5ml 1ml minicro a capillary magazi

Chuma chosungira cha micro magazi chimapangidwa ndi kapangidwe kake ndi chipewa chosindikizidwa chosindikizidwa, chubu chimatha kupewa kutayikira magazi. Chifukwa cha mano ake osiyanasiyana komanso kapangidwe kake, ndizosavuta mayendedwe ndi ntchito yosavuta, yopanda magazi imayamba.

Mtundu wa chipewa cha chitetezo chimakhala chogwirizana ndi muyezo wapadziko lonse, zosavuta kuzindikiritsa.

Kupanga Kwabwino Kwambiri m'mphepete mwa chubu ndikosavuta kwa ogwiritsa ntchito maboshoni a chubu kulowa mu chubu. Zosavuta, mwachangu komanso mothamanga, voliyumu ya magazi imatha kuwerengedwa mosavuta ndi mzere womaliza maphunziro.

Mankhwala apadera mkati mwa chubu, ndikusalala pamwamba popanda kutsatira magazi.

Imatha kusintha babcode, ndikutenthetsa chubu ndi masewera a gamma monga zofunikira za makasitomala kuti akwaniritse kuyesa kwa Asepis.

1. GEL & Chovala chogwira ntchito chubu

Geli a Geli ndi Chovala Othandizira amagwiritsidwa ntchito chifukwa cha magazi a seramu, Immunology ndi kuyezetsa mankhwala osokoneza bongo, ndi zina.

Monga kupatukana komwe ku gel kuchokera ku Japan ndi chinthu changwiro, malo okhazikika mu malo a mano, amatha kungokhala kutentha kwambiri kotero kuti gel akhazikikapo panthawi yosungirako ndi mayendedwe.

Gel adzakhazikika pambuyo pa centriftigation ndipo amapatula nthawi yayitali kuchokera ku maselo a fibrin ngati chotchinga, chomwe chimalepheretsa bwino mankhwala pakati pa seramu yamagazi ndi maselo. Kupereka kwa Prum kumayendetsedwa komanso seramu yapamwamba kwambiri kudzapezeka, zimatero zimachitika moona mtima.

Sungani Serram khola kwa maola opitilira 48, palibe kusintha komwe kumachitika pazinthu zake zazomwe zimachitika pazinthu zake komanso zotulukapo zake, ndiye kuti chubucho chimatha kugwiritsidwa ntchito mwachindunji m'masanthu achitsanzo.

- Nthawi yopumira mokwanira: 20-25min
- liwiro la centrifugation: 3500-4000r / m
- Nthawi ya centrifugation: 5min
- kutentha kosungirako: 4-25ºC

2.clot Activer chubu

Chovala chogwira ntchito chimagwiritsidwa ntchito popereka magazi katswiri wa biochemin and fumunology mu kuyendera kwazachipatala. Ndioyenera kutentha kwamitundu yambiri. Ndi chithandizo chapadera, chubu chamkati kwambiri ndizosalala kumene kukopera kwambiri kumakhala kopanda tanthauzo. Njira yamagazi imalumikizana kwathunthu ndi coagulant ndi cet mkati mwa 5-8min. Mphepo yapamwamba kwambiri imapezeka pambuyo pake, yopanda kusokonekera kwa magazi a magazi, hemolysis, kupatukana kwa mapuloteni a fibrin, etc.

Chifukwa chake seramu imatha kukwaniritsa zofunikira za chipatala chachangu komanso mayeso adzidzidzi.
- Nthawi yopumira mokwanira: 20-25min
- liwiro la centrifugation: 3500-4000r / m
- Nthawi ya centrifugation: 5min
- kutentha kosungirako: 4-25ºC

3.dta chubu

Edta chubu chimagwiritsidwa ntchito kwambiri mu hematology, ofananira ndi magazi, gulu la magazi komanso mitundu yosiyanasiyana ya zida zoyeserera magazi.

Imateteza kwambiri khungu la magazi, makamaka kuteteza magazini ya magazi, kuti iyime bwino kusonkhanitsa magazi kwa magazi ndikupanga mawonekedwe ndi kuchuluka kwa maselo am'magazi osawoneka bwino.

Zovala zabwino kwambiri ndi njira yapamwamba kwambiri imatha kupukutidwa mosiyanasiyana pamtunda wamkati mwa chubu, motero mawonekedwe a magazi amatha kusakaniza kwathunthu ndi zowonjezera. EDTA Anticoagulant Plasma amagwiritsidwa ntchito polowerera nkhani ya tizilombo tambiri, parasite ndi bacteria molekyu, etc.

4.dna chubu

1.the magazi a RNA / DNA chubu yolumikizidwa ndi reagent wapadera kuti muteteze RNA / DNA ya zoyesayesa kuti zisawonongeke

2.Magazi a magazi atha kusungidwa kwa masiku atatu pa 18-25 ° C, kusungidwa kwa masiku 5 pa 2-8 ° C, mokhazikika kwa osachepera miyezi iwiri ku -20 ° C mpaka -70 ° C

3.Vasy kuti mugwiritse ntchito, ingotchera magazi a RNA / DNA chubu nthawi ya 8 pambuyo pa kusonkhanitsa kwa magazi

4. Momwemonso magazi atsopano a anthu ndi zinyama, osayenera magazi a nthawi ya nthawi & magazi komanso magazi a nkhuku & nyama zina

Kutolera, kusungidwa ndi mayendedwe a mafupa onse a RNA / DNA

6. Kunja khoma la chubu ndikwanthawi yapadera popanda Rnase, DNASE, onetsetsani kuti mawonekedwe a zigawo za acidic acid

7.Konse ku Misa ndi kuchotsera mwachangu kwa zonena, kukonza bwino ntchito ya labotale

5.derr chubu

Ø13 × 75mm esr chubu imagwiritsidwa ntchito mwapadera m'magazi ndi kuchuluka kwa errythrocyte sedium stopleation 1 gawo lamagazi 4, ndi njira ya ku Western.

6.glucose chubu

Glucose chubu imagwiritsidwa ntchito poyeserera magazi chifukwa cha shuga, kulekerera shuga, erythrocyte elmoghores, anti-alkali hemoglobin ndi lactate. Sodium yowonjezera ya sodium imalepheretsa kagayidwe ka shuga wamagazi ndi sodium heparin bwino amathetsa hemolysis.

Chifukwa chake, mawonekedwe oyambilirawo amakhala nthawi yayitali ndikutsimikizira data yokhazikika ya shuga yamagazi mkati mwa maola 72. Wowonjezera posankha ndi sodium fluoride + sodium heparin, sodium fluoride + edta.k2, sodi fluoride + edta.na.

Liwiro lothamanga: 3500-4000 r / m
Nthawi ya centrifugation: 5min
Kutentha kovomerezeka: 4-25 ºC

7.heparin chubu
Heparin chubu amagwiritsidwa ntchito poyesa magazi poyesa matenda am'magazi, miyambo yadzidzidzi yam'magazi ndi zosokoneza magazi, ndi zina zosokoneza m'magazi, sizingapangitse Hevocyte. Kupatula apo, ili ndi mawonekedwe a plasma yofulumira ya plasma ndi mitundu yosiyanasiyana yogwira ntchito komanso kugwirizana kwambiri ndi index index.

The Anticoagulant Heparin imayendetsa fibrinolysin, ndikuletsa thromboplastin, kenako ndikukwaniritsa bwino pakati pa fibrinogen ndi fibrin, wopanda fibrin muyeso woyendera. Zolemba zambiri za plasma zimatha kubwerezedwa mkati mwa maola 6.

Lithiamu heparin sikuti mawonekedwe a sodium heparin, komanso amatha kugwiritsidwa ntchito mu kuyesa kwa inicroelement popanda zotsatira pa sodium ion. Kuti akwaniritse zosowa zosiyanasiyana za labotale, Kangjian amatha kuwonjezera geyi ya plasma popanga magazi apamwamba kwambiri.

Liwiro lothamanga: 3500-4000 r / m
Nthawi ya centrifugation: 3min
Kutentha kovomerezeka: 4-25ºC

8.pt chubu

PT chubu imagwiritsidwa ntchito pakuyesa kwa magazi ndikugwiritsa ntchito matenda a fibrinolytic (PT, TT, TT, APT ndi Fibrinogen, etc.
Chigawo chosakanikirana ndi 1 gawo la citrate mpaka magawo 9 magazi. Chiwerengero cholondola chimatha kutsimikizira mphamvu ya zoyeserera ndikupewa kusokonekera.

Pamene sodium citrate imakhala ndi vuto lalikulu kwambiri, limagwiritsidwanso ntchito posungira magazi. Chitani mphamvu yokwanira magazi kuti muwonetsetse zotsatila zolondola. PT chubu ndi ma deck owirikiza ndi malo ocheperako, omwe angagwiritsidwe ntchito kuwunika mayeso a v wf, f, ma platelet amagwira, heparin mankhwala.